TABLE 1.
Absolute quantification of Calvin-Benson cycle protein subunits in the Chlamydomonas UVM4 strain.
| Protein | Peptide | amol/cella | n | amol/cellb | % of total cell proteinc | d::pPop rank/score |
| rbcL | DTDILAAFR | 37.7 ± 6.6 | 24 | 36.2 | 6.88 | 1/1.0 |
| LTYYTPDYVVR | 35.9 ± 5.6 | 24 | 2/0.73 | |||
| FLFVAEAIYK | 37.5 ± 4.6 | 24 | 3/0.68 | |||
| RBCS | AFPDAYVR | 29.0 ± 7.2 | 11 | 24.6 | 1.45 | 1/1.0 |
| AYVSNESAIR | 22.2 ± 3.1 | 24 | 2/1.0 | |||
| LVAFDNQK | 29.3 ± 4.6 | 19 | 3/0.82 | |||
| PGK1 | ADLNVPLDK | 1.6 ± 0.4 | 24 | 2.0 | 0.31 | 3/0.83 |
| LSELLGKPVTK | 2.1 ± 0.4 | 23 | 25/0.24 | |||
| TFNDALADAK | 2.4 ± 0.4 | 24 | 11/0.65 | |||
| GAP3 | AVSLVLPSLK | 6.8 ± 1.5 | 24 | 6.6 | 0.91 | 10/0.45 |
| VLITAPAK | 6.9 ± 1.1 | 12 | 1/1.0 | |||
| TPI1 | LVDELNAGTIPR | 0.3 ± 0.1 | 23 | 0.3 | 0.03 | 1/1.0 |
| SLFGESNEVVAK | 0.4 ± 0.2 | 20 | 3/0.47 | |||
| FBA3 | ALQNTVLK | 11.5 ± 2.5 | 24 | 10.1 | 1.4 | 4/0.50 |
| SVVSIPHGPSIIAAR | 8.8 ± 1.5 | 24 | 1/1.0 | |||
| FBP1 | IYSFNEGNYGLWDDSVK | 1.9 ± 1.3 | 24 | 0.5 | 0.08 | 12/0.26 |
| TLLYGGIYGYPGDAK | 0.5 ± 0.1 | 23 | 7/0.48 | |||
| VPLFIGSK | 0.2 ± 0.04 | 12 | 1/1.0 | |||
| SBP1 | LLFEALK | 1.3 ± 0.3 | 12 | 1.2 | 0.15 | 2/1.0 |
| LTNITGR | 1.1 ± 0.3 | 11 | 9/0.52 | |||
| TRK1 | FLAIDAINK | 3.2 ± 0.8 | 23 | 2.0 | 0.53 | 2/0.90 |
| NPDFFNR | 1.5 ± 0.4 | 19 | 4/0.66 | |||
| VSTLIGYGSPNK | 1.9 ± 0.5 | 24 | 5/0.56 | |||
| RPE1 | FIESQVAK | 0.4 ± 0.1 | 8 | 0.4 | 0.04 | 3/0.80 |
| GVNPWIEVDGGVTPENAYK | 1.2 ± 0.4 | 21 | 5/0.66 | |||
| SDIIVSPSILSADFSR | 0.2 ± 0.4 | 22 | 1/1.0 | |||
| RPI1 | LANLPEVK | 0.4 ± 0.1 | 20 | 0.3 | 0.03 | 2/0.78 |
| LQNIVGVPTSIR | 0.4 ± 0.1 | 24 | 1/1.0 | |||
| PRK1 | GHSLESIK | 3.9 ± 1.4 | 18 | 1.8 | 0.25 | 11/0.26 |
| IYLDISDDIK | 1.6 ± 0.3 | 24 | 6/0.65 | |||
| VAELLDFK | 1.5 ± 0.2 | 12 | 1/1.0 |
aMean from 3 to 6 biological replicates, each measured with 1–4 technical replicates ±SD. bMedian of all values. cBased on median and using MWs of mature proteins (Supplementary Dataset 2).