FIGURE 3.

BMDM from CHIT1-OE mice and control animals were treated with 20 ng/mL IFN-γ + 100 ng/mL LPS for 0, 0.5, and 1 h. (A) Cell lysates were incubated with florescent antibodies for phosphorylated signaling molecules and expression of phosphorylated proteins was analyzed by Western blotting. Florescence intensity was measured using the Licor Odyssey cLx. All bars represent fold change in densitometric, florescence intensity between CHIT1-OE BMDM and control BMDM. Florescence signal from Western blot of phosphorylated proteins: pIκB-α, pAkt, and pERK1/2 was normalized to control protein and then compared between groups. (B) Exposure to pro-inflammatory stimuli significantly depressed pERK1 and pERK2 signaling in CHIT1-OE BMDM after 0.5 h (P = 0.0085 and 0.0219 respectively). (C) pAkt signaling was significantly different between groups after 1 h when compared to control BMDM (P = 0.0020). (D) No significant results were obtained in protein expression of pIκB-α. N = 3, *P<0.05, **P<0.01.