FIGURE 5.

(A) Cryosections of the aortic sinus from both CHIT1-OE mice and littermate controls after 12 weeks of HFD were stained with biotinylated antibodies against CHIT1. (B) Immunohistological staining for CHIT1 revealed significantly enhanced CHIT1 expression in the atherosclerotic plaques of CHIT1-OE mice when compared to control animals. Quantification represents stained area as a percentage of total plaque area (P = 0.0101). (C) Serial cryosections of the aortic sinuswere also stained with MOMA to determine macrophage content. (D) Analysis of stained area versus % total plaque area. Measurement with ImageJ software showed no significant differences in macrophage content between experimental and control animals (P = 0.1982). N = 7 per group, *P < 0.05.