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. 2020 Jun 23;11:714. doi: 10.3389/fphys.2020.00714

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Copyright © 2020 Yap, McCurdy, Alcala, Irei, Garo, Regan, Lee, Kitamoto and Boisvert.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Cryosections of the aortic sinus from both CHIT1-OE (CRE+) mice and littermate controls (CRE-) after 12 weeks of HFD were stained with Oil-Red-O to determine plaque size. (C) Analysis of plaque area is represented as a function of % total area. Measurement with ImageJ software showed no significant differences in plaque size between experimental and control animals (P = 0.5399). (B) Whole aortas were removed from both CHIT1-OE and control mice after 12 weeks of HFD. The specimens were pinned and stained with Oil-Red-O. (D) No difference was observed between groups upon quantification of plaque area as a percentage of total aortic area (P = 0.8731). N = 14 per group.