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. 2020 Jun 23;11:452. doi: 10.3389/fgene.2020.00452

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Copyright © 2020 du Preez, Breeds, Burger, Swiegers, Truter and Botha.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Comparison of the average relative expression (R mean) of DNMTs in South African RWA biotype heads. Fold changes in expression are shown relative to the SA1 samples, the expression of which was set at 1. DNMTs expression is presented when normalized against the reference genes L27 and L32, respectively, and the error bars indicate standard deviation. Different alphabetical letters indicate statistical significance (p ≤ 0.05). (B) DNA Methyltransferase protein activity (OD/h/μg) measured in South African RWA biotypes SA1 and SAM, with error bars indicating the standard deviation.