Fig. 2.

Phylogenetic analyses of the partial sequences of the BCoV S gene. The analyses involved the polymorphic region of the BCoV S gene at the nucleotide level. The gene corresponded to positions 25,006­–25,416 of the Kakegawa strain [9]. The scale bars indicate the number of substitutions per site. (a) Sixty-nine strains identified in this study are connected as “name of strain”, “isolated prefecture”, and “isolated year” by hyphens (Ex. C20-Miyazaki-2016). On the other hand, the sequences retrieved from GenBank are connected as “isolated prefecture”, “name of strains”, and “isolated year” by slashes (Ex. Tochigi/1/2001). Bootstrap values of more than 50% (1,000 replicates) are shown next to the branches. (b) Eighty-six strains identified in Japan from 1999 to 2018 and 170 strains identified throughout the world were involved in the phylogenetic analysis.