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. 2020 Jun 23;14:167. doi: 10.3389/fncel.2020.00167

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Copyright © 2020 Slaoui Hasnaoui, Arsenault, Verdier, Obeid and Kolta.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Distribution of NVsnpr neurons activated by stimulation of NVmt. (A) Schematic drawing of the brainstem slice preparation and the experimental conditions used. (B) Photomicrograph showing the position of responsive cells in NVsnpr following electrical stimulation of dorsal NVmt and their synchronous Ca²⁺ responses. Gray vertical line indicates the length of the train of stimulation. The bottom trace shows a stimulus artifact. (C) Distribution of cells responding to stimulation of both NVmt-D and NVmt-V throughout NVsnpr (Left) and count of the number of cells per division in NVsnpr (Right). (D) Heatmap representing the density of responsive neurons in NVsnpr following stimulation of NVmt-D and NVmt-V pooled together. Abbreviations: Stim, stimulation; NVsnpr, trigeminal main sensory nucleus; NVmt, trigeminal motor nucleus.