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. 2020 Jun 23;14:167. doi: 10.3389/fncel.2020.00167

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Copyright © 2020 Slaoui Hasnaoui, Arsenault, Verdier, Obeid and Kolta.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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BAPTA applications near NVsnpr neurons induce transient and recurrent Ca²⁺-responses that can sometimes occur synchronously in nearby neurons. (A) Right top: schematic drawing of the brainstem slice preparation and the experimental conditions used. Left top: photomicrograph showing the position of the responsive cells in the NVsnpr following local BAPTA application. Bottom: calcium responses of several NVsnpr neurons following the BAPTA application. Gray lines crossing several traces emphasize synchronicity between recurrent events occurring in different cells. (B) Illustration of the three main types of calcium responses observed in NVsnpr neurons following local application of BAPTA. (C) Vertical bars chart reporting the percentage (and number) of types of calcium responses observed in NVsnpr neurons following local applications of BAPTA. Abbreviation: NVsnpr, trigeminal main sensory nucleus.