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. 2020 May 5;11:9. doi: 10.1186/s43008-020-00029-9

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — MCS levels and profiles in fungal cells grown in glucose or propionate. The protein extracts from yeast cells grown on propionate (Prop) and/or glucose (Glu) were subjected to 1-dimensional (a) or 2-dimensional (b) Western Blot analysis by using polyclonal antibodies raised against rPbMCS. Secondary anti-mouse antibodies coupled to alkaline phosphatase were used and the reaction was developed with BCIP and NBT