Figure 3: CD209L and CD209 mediate SARS-CoV-2 spike-pseudotyped cell entry.
(A) HEK-293 cells expressing CD209L, CD209 or ACE2 (2X104/well, 96-well plate and 6 wells/group) were infected with pseudotyped virus (10ng/ml) or mock virus. After 24hours, cells were analyzed for luciferase activity. (B, C) CD209L/HEK-293 cells seeded in chamber slides were mock-infected or infected with SARS-CoV-2 at the indicated MOI. Cells were fixed at 1 day post infection, and stained with an antibody directed against the viral nucleoprotein, N (green). Cell nuclei were stained with DAPI (blue) or dsRNA.