Figure 1.

Comparison of two H₃R_{Nluc/Halo(618)} biosensor versions. (a, b) Representative luminescence emission spectra of full-length H₃R_{Nluc/Halo(618)} (a) and Δicl3-H₃R_{Nluc/Halo(618)} (b). (c, d) BRET changes reported by full-length H₃R_{Nluc/Halo(618)} (c) and Δicl3-H₃R_{Nluc/Halo(618)} (d) upon addition of H₃R reference ligands. All experiments were conducted in HEK293 cells transiently transfected with the indicated biosensor. Data in (c) and (d) show pooled data from three independent experiments. *p < 0.05 vs vehicle control.