Fig. 1.

Generation of iPSCs-derived DA neurons and astrocytes. a The protocol for the generation of DA neurons and astrocytes from iPSCs. b Immunocytochemistry of DA neuron-specific markers anti-TH (green) and anti-Tuj1 (red), as well as astrocytes-specific markers anti-GFAP (green) and anti-S100β (red). c Representative immunoblots for TH and Tuj1 expression in DA neurons, and GFAP and S100β expression in astrocytes. Three independent experiments were performed (N = 3). Scale bars in all panels represent 50 μm