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. 2020 Jun 29;20:184. doi: 10.1186/s12866-020-01867-8

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Formation rate and composition analysis of biofilms made by S. mutans NCTC 10449 (a, c, e) and clinical isolate 2366 (b, d, f) in BHI-sucrose medium (suc), and BHI-sucrose medium supplemented with eryhtritol (ery) or xylitol (xyl). a and b: Biofilm formation as measured in xCELLigence, the erythritol and xylitol curves significantly differed from sucrose in all time points, the line indicates the time period where there is a significant difference (p < 0.05) between erythritol and xylitol, c and d: Biofilm samples were collected at indicated time points and number of living bacteria were determined by plate counting, d and e: Amount of polysaccharides in the biofilm at different time points as measured by Anthrone method from collected biofilm samples. * significant difference (p < 0.05) between sucrose and xylitol, ^#significant difference (p < 0.05) between sucrose and erythritol, ^osignificant difference (p < 0.05) between erythritol and xylitol, **/^##/^oop < 0.01, ***/^###/^ooop < 0.001