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Primers used in this study
| Gene | Primer sequence (5′- 3′) | Annealing temperature (°C) | |
|---|---|---|---|
| gbpB | F | TCCAGCAGGGCAATGTACTTG | 59 |
| R | CCACCATTACCCCAGTAGTTTCC | ||
| gtfB | F | GACACTCCTTACCTTCAT | 57 |
| R | TTCAGCATTATCATCAGTTC | ||
| gtfC | F | GATGCTGCAAACTTCGAACA | 57 |
| R | TATTGACGCTGCGTTTCTTG | ||
| gtfD | F | TTGACGGTGTTCGTGTTGAT | 59 |
| R | AAAGCGATAGGCGCAGTTTA | ||
| gyrA | F | ATTGTTGCTCGGGCTCTTCCAG | 59 |
| R | ATGCGGCTTGTCAGGAGTAACC | ||
| recA | F | AATCACTGGCAATCTTAACTAAT | 57 |
| R | TGATGTCCGTGGCAATAC | ||
