Fig. 4.

Analysis of C-terminal sequences of AD homologs in bacteria. a Phylogenetic reconstruction of AD sequences. The phylogenetic tree was generated using MEGA 5.10’s NJ method with 100 bootstrap replications. b Multiple sequence alignment of the C-terminal regions of eight AD sequences, with three representatives (labeled a, b, c) used for subsequent investigation. c Statistical analysis of C-terminal sequence of 371 AD homologs. d GFP degradation test of three representative C-terminal peptides and the conserved degron sequence. GFP-A, GFP-B, GFP-C represent GFP fused with three C-terminal regions from (b). GFP-ADcon represents GFP fused with conserved sequence from (c). Fluorescence values were normalized to the number of cells by dividing by the OD₆₀₀. The data shown are from the mean of three biological replicates