Fig. 5.

Investigation of degradation mechanism of AD degron. a Effects of protease deletion on GFP degradation with data normalized by OD₆₀₀. b Diagram illustrating the recognition and degradation process of AD degron-tagged protein. GFP-A, GFP-B, GFP-C and GFP-ADcon refer to Fig. 4d. AD degron can be recognized by the ClpA and Lon protease complexes. ClpA-bound GFP will be unfolded and translocated into ClpP for subsequent degradation. Lon-bound GFP will be unfolded and degraded simultaneously in itself. Fluorescence values were normalized to the number of cells by dividing by the OD₆₀₀. The data shown are from the mean of three biological replicates