Fig. 4.

Exosomal miR-200c-3p expression did not change HCT-116 proliferation. Cells were treated with anti-miRNA or miRNA mimics for 24 h, and then treated with LPS and EdU, together with mock or exosome inhibitor GW4869 for additional 24 h as indicated in the Fig. a. Expression of miR-200c-3p in the exosomes of HCT-116 cells without transfection, or after transfection with anti-miR-NC (negative control) or anti-miR200c-3p (n = 3). N.S., not significant; ** p < 0.01; b Representative images of anti-miRNA-transfected HCT-116 cells labeled with EdU (red) and Hoechst 33342 (blue) 24 h after drug treatment. Scale bar: 100 μm; c Statistical analysis of EdU+/ Hoechst 33342+ cell ratio between groups with indicated treatment in (B) (n = 3). Two-way ANOVA was performed. N.S., not significant; d Expression of miR-200c-3p in the exosomes of HCT-116 cells without transfection, or after transfection with miR-NC (negative control) mimic or miR200c-3p mimic (n = 3). N.S., not significant; ** p < 0.01; e Representative images of miRNA mimic-transfected HCT-116 cells labeled with EdU (red) and Hoechst 33342 (blue) 24 h after drug treatment. Scale bar: 100 μm; f Statistical analysis of EdU+/ Hoechst 33342+ cell ratio between groups with indicated treatment in (E) (n = 3). Two-way ANOVA was performed. N.S., not significant