FIGURE 4.

PANX1 expression appears to be both necessary and sufficient for intracellular Ca²⁺ responses. (A) si-PANX1 RNA treatment in PC3 cells reduced Ca²⁺ responses compared to si-negative RNA (scramble) as a control. (B) Quantitative cell response index (CRI) values of the si-PANX1 RNA treatments relative to the control. n = 3. Error bars, s.e.m., exact p-values by a two-tailed t-test. (C) Fluorescence patterns in cells that first responded to the FUS stimulus after the treatments. One representative cell (top) is shown with fluorescence patterns in ten cells (bottom). (D) HEK293T cells transfected with WT PANX1 or mt PANX1^1–89-mRFP (mt PANX1-mRFP) constructs showed Ca²⁺ responses while control HEK cells transfected with dsRED construct have no FUS-induced Ca²⁺ response. (E) Quantitative CRI values of the transfected cells. n = 3. Error bars, s.e.m., ANOVA, Dunnet’s correction, exact p values.