FIGURE 6.
Effect of inhibitors of cytoskeletal support and actomyosin on FUS-induced Ca2+ responses in PC-3 cells. The responses are represented when cells were treated with mock, 2 μM CytochalasinD (CytoD), 5 μM ML-7, 5 μM Blebbistatin (Bleb), and 1 μM Nocodazole (Noc). (A) The percentage of responding cells over time. (B) The time at which each cell first responded to the stimulus. (C) Percentage of responding cells after the treatments. Cytoskeletal support and actomyosin did not affect FUS-induced calcium responses. None of them reduced the calcium responses, suggesting a distinctiveness of FUS. n = 4. Error bars, s.e.m., NS, not significant, by a one-tailed t-test.