. Author manuscript; available in PMC: 2020 Aug 2.

Published in final edited form as: J Proteome Res. 2019 Jul 19;18(8):3032–3041. doi: 10.1021/acs.jproteome.9b00145

Table 1.

Summary of LC-MS/MS method

LC Instrument	Waters ACQUITY UPLC
LC column	Waters 1 mm × 15 cm, 1.8 μm CSH (Part #186006935)
Mobile phase (MP)	MPA, 0.1% formic acid/99.9% H₂O MPB, 0.1% formic acid/99.9% MeCN
Gradient	0–15 min, 3–25% MPB 15–15.5 min, 25–90% MBP 15.5–17.5 min, 90% MBP 17.5–18 min, 90%–3% MPB 18–20 min, 3% MPB
Flow rate	100 μL/min
Injection volume	14 μL (plasma); 8 μL (whole blood)
Divert solvent (whole blood only)	0.1% FA/49.9% H₂O/50% MeCN, 100 μL/min; 0–7 min and
MS Instrument	Thermo Q-Exactive HF-X
MS method	PRM, 0–20 min
MS2 resolution	60,000
AGC target	1E5
Max injection time	300 ms
Isolation width	0.7 m/z
Normalized collision energy	27