Figure 6.

Iron supplementation does not adversely affect macrophage or neutrophil bacterial killing. Effect of 50 μM ammonium iron(III) citrate supplementation on in vitro splenic CD11b⁺ macrophage intracellular killing (A) and splenic CD11b⁺ neutrophil total killing (B) in 2- to 4-month-old adult mice using the gentamicin protection assay. Error bars indicate ±1 standard error of the mean, n = 4 per group, Two-way analysis of variance Tukey multiple comparisons test. Results represent 2 independent experiments. Abbreviations: CFU, colony-forming units; FAC, ammonium iron(III) citrate; PMN, polymorphonuclear cells.