Table 2.
Nested PCR (nPCR) and indirect immunofluorescence assay (IFA) results for anaplasmosis and rickettsiosis
| Date | Anaplasmosis | Rickettsiosis | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| nPCR | IFA | nPCR | IFA (R. conorii) |
IFA (R. japonica) |
IFA (R. montanensis) |
|||||
| IgG | IgM | IgG | IgM | IgG | IgM | IgG | IgM | |||
| 19 June |
groEL (+) ankA (+)16S rRNA (−) |
< 1:16 | < 1:16 |
sca1 (−) ompA (−) |
< 1:16 | 1:256 | 1:128 | < 1:16 | 1:32 | < 1:16 |
| 22 June | < 1:16 | 1:16 | sca1 (−) | < 1:16 | 1:512 | 1:128 | < 1:16 | 1:32 | < 1:16 | |
| 28 June |
groEL (−) ankA (−) |
< 1:16 | 1:32 | sca1 (−) | 1:64 | 1:256 | 1:64 | < 1:16 | 1:32 | < 1:16 |
nPCR nested PCR, IFA indirect immunofluorescence assay