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. 2020 May 21;9(5):bio051680. doi: 10.1242/bio.051680

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© 2020. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 1. — DJ-1 induces EMT in colorectal cancer cells. DJ-1 was stably overexpressed in SW480 cells. (A) Wound-healing migration assay of indicated time. Scale bars: 250 μm. (B–D) Quantitative analysis of cell migration and Matrigel invasion assays. Migration was analyzed at 24 h, invasion at 48 h. All data were from at least three independent experiments and shown as mean±s.d. Scale bars: 50 μm. (E) Expression of DJ-1, E-cadherin and Vimentin was examined by qRT-PCR. Y-axis means relative expression to control group. (F) Expression of DJ-1, E-cadherin and Vimentin was examined by immunoblot. (G) Expression of E-cadherin and Vimentin was examined by immunofluorescence. Scale bars: 50 μm. *P<0.05, **P<0.01, ***P<0.001.