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. 2020 May 21;9(5):bio051680. doi: 10.1242/bio.051680

Fig. 3.

Fig. 3.

FGF9 is required for DJ-1-induced and Wnt signaling-mediated EMT. (A) Western blot analysis of FGF9 expression in SW480 cells stable transfected with DJ-1 cDNA or vector. (B) Western blot analysis of FGF9 in SW480-DJ-1 cells treated with or without 100 μM Sulindac for 36 h. (C–H) SW480-DJ-1 cells were transfected with specific FGF9 siRNA. (C) Wound-healing migration assay of indicated time. Scale bars: 250 μm. (D–F) Quantitative analysis of cell migration and Matrigel invasion assays. Migration was analyzed at 24 h, invasion at 48 h. All data were from at least three independent experiments and shown as mean±s.d. Scale bars: 50 μm. (G) Expression of FGF9, E-cadherin, and Vimentin was examined by immunoblot. (H) Expression of E-cadherin and Vimentin was examined by Immunofluorescence. Scale bars: 50 μm. **P<0.01.