Fig. 3.

IPSG components colocalize in arsenite- and rapamycin-induced IPSGs. Super resolution micrographs of progenitor cells stained for (A–C) RIN::HA (red), FMRP (green) and DAPI (blue) or (D–F) RIN::HA (red), DCR2 (green) and DAPI (blue) after treatment with (A,D) KRB, (B,E) 1 mM sodium arsenite in KRB, or (C,F) 1 mM rapamycin in KRB. Insets are enlarged views of boxed regions in the main panels. RIN::HA and FMRP, and RIN::HA and DCR2 show only occasional colocalization under control conditions (A,D). However, after arsenite or rapamycin treatments, RIN::HA and FMRP colocalize with each other (B,C). In contrast, DCR2 does not colocalize with RIN::HA following stress induction (E,F).