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. Author manuscript; available in PMC: 2021 Mar 15.
Published in final edited form as: Mol Cell Endocrinol. 2020 Jan 11;504:110703. doi: 10.1016/j.mce.2020.110703

Figure 1:

Figure 1:

Effects of 17β-Estradiol (E2) or Selective Estrogen Receptor (ER) Agonists on Glycogen Synthase (GS) Expression in Female or Male Rat Hypothalamic Astrocyte Primary Cultures. After incubation of female [Panel 1A; p < 0.0001] or male [Panel 1B; p < 0.0001] rat hypothalamic astrocytes with varying (100 pM – 100 nM; gray bars) E2 concentrations or media lacking E2 (white bar), cell lysates were analyzed by Western blotting for GS protein expression. Data depict E2 effects on mean normalized GS protein optical density (O.D.) measures ± S.E.M. Panels 1C [female; p < 0.0001] and 1D [male; p < 0.0001] illustrate effects of E2 (solid gray bar) versus the selective ERα agonist PPT (diagonal-striped gray bar), the ERβ agonist DPN (horizontal-striped gray bar), membrane impermeable E2-BSA conjugate (cross-hatched gray bar), or the GPER agonist G1 (vertical-striped gray bar) on GS protein profiles. *p < 0.05; **p < 0.01; ***p < 0.001.