FIGURE 5.

CTBP2 labeling in INL and GCL. Retinas from wt, Tulp1-/-, Rho-/-, and Rds-/- mice were taken at p5, p8, and p14 (n = 5–6). (a–l) Sections were labeled with CTBP2 immunocytochemistry (purple) and counterstained with DAPI (blue; overlaid on the right). CTBP2 label intensities (n = 4) were quantified in the inner nuclear layer (INL; m) and ganglion cell layer (GCL; n) from microscope images using cellSens software, and results are given in bar charts; bars represent mean + SD. NBL, neuroblast layer, ONL, outer nuclear layer. Scale bar (l): 50 mm. *p < 0.05; **p < 0.01; ***p < 0.001 (ANOVA). Black stars above bars refer to differences between IRD and wt mice, purple stars above bars refer to differences between Tulp1-/- and the other IRD mice; black stars within bars refer to a difference compared to the previous time point.