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. 2019 Feb 19;221(6):867–877. doi: 10.1093/infdis/jiz081

Figure 6.

Figure 6.

Sera from TAK-003-vaccinated patients prevents dengue virus (DENV)-2 NS1-induced sialic acid and heparan sulfate degradation on human pulmonary microvascular endothelial cells (HPMEC). The effect of DENV-naive (1023014) or preimmune (1052010) sera on DENV-2 NS1-induced disruption of sialic acid (Sia) and heparan sulfate (HS). A, The integrity of the endothelial glycocalyx-like layer on HPMEC was assessed by the surface expression of sialic acid (red, top row) and heparan sulfate surface expression (green, bottom row) at 6 hours posttreatment with serum (as indicated) + DENV-2 NS1 (5 µg/mL) at 37°C, as visualized via confocal microscopy. Nuclei were stained with Hoechst (blue). Images are representative of 1 independent experiment performed in duplicate (×20; scale bar, 50 μm). B and C, Quantification of mean fluorescence intensity (MFI) of (B) sialic acid and (C) heparan sulfate expression from (A) and Supplementary Figure 5 from 1 independent experiment. Values normalized to MFI from the NS1 + positive control serum group (represented by dotted line at 100%) and expressed as percentage of control. Error bars indicate SEM.