Figure 2.

Segmentation of vessel layers. (A−C) The upper row of images shows the en face infrared projection of the tissue slab containing the specific vessel layer as defined by the structural OCT (D−F). (G−I) The third row shows en face angiography projection for the SVC (G), ICP (H) and DCP (I). (J-L) Large vessels were masked using ImageJ prior to analysis. (M-O) Vessel layers were analyzed using AngioTool to return the % area within the region of interested occupied by vessels (highlighted in green, vessel center shown by red traces).