Figure 4.

EMP2 is expressed in the neuroretina in pathologic OIR. In situ hybridization was used to detect Emp2 mRNA expression. Representative images of P12 WT mouse pup retinal sections demonstrate Emp2 mRNA expression within the RPE layer in normoxia conditions. However, in OIR mice, Emp2 mRNA expression is detected at P12, now in the ONL and INL of the neuroretina, and its expression increased at P17 (peak neovascularization). Isotype-matched negative control is shown for comparison. Scale bars represent 50 µm in the top row. Arrows indicate positive staining (fuchsia) in inset boxes shown in the bottom row. GCL, ganglion cell layer.