Fig. 1.

The clonoSEQ Assay Processg: DNA is extracted from the patient sample, and the CDR3 regions of B- and T-cell receptors are subject to multiplexPCR to amplify their unique VDJ or VJ sequences. Amplified DNA undergoes a second round of PCR to add index sequences to prepare for NGS, which is performed via synthesis. The resulting sequences are processed by bioinformatics software to ensure accuracy of results