Table 2.

LoD and LoQ of the clonoSEQ Assay by MRD cell counts and MRD frequency

Measure	Malignant cellsa (95% CI)	500 ng DNA input frequency (95% CI)	20 μg DNA input frequency (95% CI)
LoD	1.903 (1.75–2.07)	2.26 × 10− 5 (2.01 × 10− 5–2.53 × 10− 5)	6.77 × 10− 7 (6.02 × 10− 7–7.61 × 10− 7)
LoQ	2.390 (1.90–9.14)	2.39 × 10−5 (2.26 × 10− 5–7.01 × 10− 5)	1.76 × 10−6 (6.77 × 10−7–4.09 × 10− 6)

CI confidence interval, LoD limit of detection, LoQ limit of quantitation, MRD minimal residual disease

^aCalculated from samples with 500 ng and 20 μg of DNA input