Figure 2.

Pravastatin modulates phagosome maturation in a dose-dependent manner. Murine bone marrow-derived macrophages were treated with the indicated pravastatin concentrations (or water as a vehicle control) for 2 days before assay start. Pravastatin treatment decreases phagosomal acidification (A) and proteolytic activity (B) in a concentration-dependent manner, phenocopying phagosome maturation profiles of macrophages activated by interferon-γ and lipopolysaccharide (C and D). Carboxyfluorescein (A and C) or DQ-BSA/AF594 (B and D) beads were added to the cells, or to wells containing only media, and fluorescence tracked with a microplate reader over time. Data are shown as means ± standard deviation from 3–4 wells.