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. Author manuscript; available in PMC: 2021 Jun 4.
Published in final edited form as: Mol Cell. 2020 May 27;78(5):941–950.e12. doi: 10.1016/j.molcel.2020.05.008

FIGURE 2: Germ granule proteins are more mobile than germ granule mRNAs.

FIGURE 2:

See also Figure S3 and Table S2. A,B Fluorescence recovery of Vasa:GFP (A) and nos MS2-MCP:GFP (B) after photobleaching an ROI (magenta square) within germ plasm. C Labeling of nos with MS2-MCP:GFP. D Recovery of Vasa:GFP (mean±STDEV, gray bars) of 5 normalized recovery curves. Magenta line: fit to the data. E Kinetics of fluorescence recovery of core granule proteins and nos. The half-time to recovery (t1/2 (s)) and the percent mobile fraction of the population that exchanges between granule and the intergranular space is shown. Mean±STDEV of 5 Osk:GFP, 5 Vasa:GFP, 12 Tud:GFP, 9 Aub:GFP and 5 nos MS2-MCP:GFP recovery curves. Scale bar = 10 μm.