KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, Peptides, and Recombinant Proteins
TRIzol reagent	Invitrogen	15596018
Acid-phenol:chloroform	Invitrogen	AM9720
Chloroform	Fisher	AC40463–5000
Isopropanol	Fisher Scientific	A416–500
RQ1 RNase-Free DNase	Promega	M6101
Oligo dT(20) primer	Thermo Fisher Scientific	18418020
SuperScript® III Reverse Transcriptase	Life Technologies	18080–044
YBR Green reporter dye	Roche	04707516001
Tissue freezing medium	General Data	TFM-C
Poly-L-lysine solution	Sigma	P4707–50ml
Epoxy	Devcon	00470740
Catalase	Sigma-Aldrich	C3115–50MG
Glucose Oxidase	Sigma-Aldrich	G2133–10KU
IRF670 plasmid	Addgene	45457
Critical Commercial Assays
AlexaFluor 488 oligonucleotide Amine labeling kit	Life Technologies	A-20191
MicroSpin G-25 Columns	GE Healthcare	27-5325-01
384-well qRT-PCR plates	Roche	04729749001
Plastic disposable molds	Fisherbrand	22-363-552
Cryostat	Leica	CM 3050 S
Low profile blades	Accu-Edge	4689
Experimental Models: Organisms/Strains
w1118 (“wild type”)	Bloomington Drosophila Stock Center	5905
pFlyFos-Osk:sfGFP	(Jambor et al., 2015, Trcek et al., 2015)	N/A
y,w; P[E GFP-vas w+]cyIII	(Trcek et al., 2015)	N/A
UASp-GFP-Aub	(Webster et al., 2015)	N/A
GFP-Tud	(Zheng et al., 2016)	N/A
;;P{GAL4::VP16-nos.UTR}/TM3Ser	(Pae et al., 2017)	N/A
w; matα-gal4;PrDr/TM3	(Pae et al., 2017)	N/A
w; P(EPgy2)gclEY09611/CyO; nosGal4VP16 (w+)/TM3	(Cinalli and Lehmann, 2013)	N/A
w; P(EPgy2)gclEY09611/CyO; nosGal4VP16 (w+)/TM3	(Cinalli and Lehmann, 2013)	N/A
nos-(MS2)18	(Sinsimer et al., 2013)	N/A
hsp83-MCP-GFP	(Forrest and Gavis, 2003)	N/A
nos-tub B,C, D chimeras	(Gavis et al., 1996)	N/A
nos RNAi y1 sc* v1; P{TRiP.HMS00930}attP2	Bloomington Drosophila Stock Center	33973
CycB RNAi y1 sc* v1; P{TRiP.HMS02163}attP2/TM3, Sb1	Bloomington Drosophila Stock Center	40915
mCherry RNAi y1 sc*v1 sev21; P{y[+t7.7] v[+t1.8]=UAS-mCherry.VALIUM10}attP2	Bloomington Drosophila Stock Center	35787
w[*]; P{w[+mC]=GAL4-slbo.2.6}1206 P{w[+mC]=UAS-GFP.S65T}T2/CyO	Kyoto Stock Center	108–787
ry nosBN e / TM3 Sb e	(Wang et al., 1994)	N/A
sp/CyO; nosBN,Vasa:GFP/TM3 Ser	This study	N/A
nosDef (Df(3R)DlFx3/TM3 Sb, Ser)	(Forbes and Lehmann, 1998)	N/A
moesin-GFP ORF tagged with nos 3′UTR	(Rangan et al., 2009)	N/A
EGFP ORF-tagged with gcl 3′UTR	(Rangan et al., 2009)	N/A
yw; P(w+EGFP-nos)11; P(w+EGFP-nos)5	(Forrest et al., 2004)	N/A
nosp-5′gcl-EGFP-3′gcl and nosp-5′pgc-EGFP-3′pgc	(Eagle et al., 2018)	N/A
smFISH Probes (5′ to 3′)
aret cgtatccagacggaagcagg, aacatcttgatgttgtccgg, cgtactcctcgaacatctcg, cgcagaacgttgatcgagtg, aaagcagcaacccttgctaa, gcggcgtgtctcgtataaaa, attatgcaaagcatcctggg, acatcccatttagcgtctta, gggcttcatttgaatgggat, cattgcgattctcgctatcg, atacccacaaagagtttgcg, ctcgttcagcttcttgttta, ccgtggacttcaaatagctt, ttggtggcgaatgtgacgaa, ctttaatggccgaaatggca, atgatcttgttctggctcag, gtatcggcgaacttgacgac, ttccagagattggcctgaat, aggtatgttgatgttgctgg, aatggcatctgcgccgagaa, ctgcagcagttgaatggagg, gtgagggcttgcaacaactg, catcggggtcaaaagtcctg, aatggccgctagattctgga, cattcgagagactcggctgc, gatgccattggattcggatc, ggcggctgttgacatatatg, gcgaagtggacaatgcactg, gcggcagatggtagatgaaa, agatccgtatcggtgaactc, cgagatcacattgccgaagg, ctcgtctgtttgtcgatgaa, aaacccgaagcacttcgaca, agtccggattgtcgaaggag, ttcttcagctgcaccttgag	Stellaris smFISH probes	N/A
tao1 ttgtagccggacatctgttc, taaagttgtggagcagcgtg, tgctgatgcttcgtctcaag, tttgtgtagtttcttctcgg, ttctcctgctttagcgtaat, gtcgactcatccatggagag, tgcgattgcagggtaagatc, agctcgatgtactgcttctg, atcatccgtttgcgcttgaa, gtggtgcttaagaagcatgg, tgttgcttgtttatctgctc, ttttgattatgcaactcggt, tcttgatgcggtccatgtag, cttttgcttcaagctcttcg, aactgcttgcgtatctggag, tgtagcgtttgtattgcttc, tttggtgtcgtctgcagtac, ttaatgacctccttctgttg, cgatgcttctcttccttcag, atgctttgctcgtactgttc, ctgactttggaacatgtccg, ggctttcgtctagtttgtag, gtacgttggcactcaatcac, ttcttgttcttgttctggta, cgattctcgagctcacgacg, tccatcttattctcgagcag, ctggttgaattgctgcaact, tagtatgcttctcgtgtttc, ttcgttatcaaatgcttcca, tgctgaaacctagggcaatt, tcgggatacctcaatcagag, attgaaccagacagacttcc, gttgaactattgctatgggc, atagcgaaccagccggaaaa, tggcattgttgttgtctatg, actaaacacttgttgtgggg, tcgcactgttggctttataa, gatttctgttgcttgtgtcg, gctctattgatgtcttagcg, tgtttctgctgctattgttt, aaacattgctagctagccat, gtgtgtcttctttctagttc, catgtgtgttttgggttgtg, ccaatccgataattgatcgc, ggttctcatgttttcctaag, tattgcagctttagcttgtt, ccgatcgagtttcctttaat, atgttagtatcttgttgcca	Stellaris smFISH probes	N/A
pum ctgcgagtagagattctgtt, tcaacgagctcgtgttcaag, tccgaacatattgttggcag, gggattcgagaagatcgagc, cttcgagaaggcgagatctt, gagatcgcgtagctgaagat, cctgtgagaactccacaatg, ttctgttggataaaccgcga, atctcgctgaacaccatttg, ctggatgacatagttgccaa, ttgacgcatttcagcacatg, cacatgattgccattctgat, acgcactcaatgcacttttg, ttgatgatgaactgcagcgc, tgggtgcttagcgagtaaac, ctcaaggattctctggatca, ctcatgcagttcgtccaaaa, acatagttgccatattggtc, gtgttcaagcacatgctgaa, agaatcgacttatcctcctg, tgatagcaccagaactttgc, acgtttgaggcgaacttgtg, catgggtaacacatttctcc, cttcatcatcacgtgcaacg, accacatagttggcatactg, aacttggcattgatgtgctt, gggattggttatcttcatgt, accgaacaaatggtgggtga, gcctggcttaatttgttatt, tgttaagctctacagcttct, ttatcgctgcttttccaata, caaatcttcgagtgcagctc, tgtgtgttttactatcctct, aagctcttttgcttttttgc, ttatgcgagcgcattttaca, tttcatctttgcggttcatg, gatcgcgatttactacgcat, tttcttttttttcagctcgc, tcgtaggctaattcatgcag, aatggttcctctgtctgaac, gttatccctcgtatatatgt, ctgctggctccaaaaacatt, tctctgttttcattgtgtct, attgtgtgggtgtatgcaac, tgaatgggctgaagagtcgt, gtttgttgtttggggttttt, gaccaatatccatgcagatc, tttggcttacttggctaaca	Stellaris smFISH probes	N/A
eIF4G2 attgttgaaagtggggtggt, cgttagtgggaaaggtcaca, aagctgtttctgttgtggag, actgggaatgccaaagttgt, ccaaaataggggtcgtcatc, caagtgcggagcaaatgtgt, ctgttgttactgacggagtt, ggtactattgtcatccagag, acaataacgatgaccccgag, ctgatctgatctttatccgt, ctttgtttgttggccaattt, agctgtggtaactatcgact, aatactcgacttcctgttgg, gtggtaatgctggagttgat, ttctgttttttcttctccag, gcattgttgctatttgtgtt, gaattgaagctgttgcgtct, gagctgcatatgaaacctgt, agcgatagtttttgggaacc, ggcacatagtccaatgattc, cttaagcagcacctcaaagt, catttgtcattttagcctca, ctgaatgcgggtgatcaacg, tttgcgttggcatcatttac, cgactgcagcttgaaaagct, aacaaatgacccactgtggt, tcttgcatcataaaccgcac, tttggcatcttctgcatgaa, atctatgcttagggtctgat, agcaattgaatcgtcggtgg, aacaaggatgtaggcggagg, gaatgcgtcatttacttcca, gtgcaaatagtccgtgagta, ccaaacatcttttaggtcca, ctgtacaaactcccgatact, gagtgcaacagctacggaag, tcgatacacagtcgcagtag, tctatgcaagcaatctccag, tacagaacgctgaacgtgct, tagcttataccacttgtcga, tcaatgaatgggcgacggta, agtttgtggttggttgtttg, tggtttcaaacgatgttgcc, ctgatctagtcatcgtgcaa, tctatacccagttcacattg, ttcgtatttgttttgagcca, gctctttgtttttgcttttt, ttgcattgggttttcattgt	Stellaris smFISH probes	N/A
orb tgcaaattctgcagtttggc, aggagctttagcgtgttgat, ttgatcagcggcatttgttg, cgctcatcaagttaccaaga, ccatttctgtcatcagtgat, ccatcgttggtggttataat, gcagccgaactcttatagaa, ttgagcatacggtgcgaacg, cgaggaagaccttgggagaa, ctcactaatatcccagggaa, gcttgaagatctggatgagc, gccactccactttaatagat, taaacataacccttgggctg, gcttgtccgattcaaagatt, gccgaaagtaatgccttgac, agaatcatccacctgaagca, agaagtagttcctaccacag, atacgccgcgaagagatttt, tgacttcaacatccttggac, gagtcagcgataatccaagg, ggagctggatcgcacaaaat, caaacacggttttcgttggg, atccattatatttcccaagc, atctataccagcatacagca, cgatcgggtacttgtacttg, ttgctaaatgtcacacgtcc, cagctttcatataggagcga, taatctcgataaaggcggcc, cttcttggtgaacttcgtgg, caccgcatatggaacatagg, attccctacaatagtaggga, gcagaagtatcggaagcacg, ttgacgatgtcacagctgtg, acttggagttgcgagtcaag, tcgacgaagatggtccgatg, ggccagagaagggtaacgaa, cattctgttgtccctgatac, catcacgttgagactgtggg, ttaccattgatgtagcagcg, tgaatttgccgctgctgaag, atgctgttgacgctgtattc, agccgtaacagtctagatcg, catccgttaaaacggtgtgg, gtccaaagtctcacacatta, catatttctacgtcgcctac, ccatcggctgcaagaaaact, taatgacgatgatgagcccg, agcttcatattgtaccgata	Stellaris smFISH probes	N/A
CG18446 acgtttctctgcggactaaa, ttaaattcactagggtggcc, caactgttctgtaggatcgc, ctcttcggttggtggaatta, tatgatctccacatcgctat, gtggtgatgatggttcatga, ggtatggcggatggaatttg, cagtgggtgaaaatggtggg, gttccttcgggaacatttat, aggtagcacggaacacagag, atcattgaagggctgtttgc, gtgttattccaggcgttaat, acaaagggtgttatcgtcgg, ataagtgctgatctgtggga, attgtgatggtgatccatgt, cgagcggcggtgagtaaaaa, atgaaccgcaggcatttgaa, ggtggtatctgaatcggatg, tatgctgagcggtcatatac, gccgatgacctaaaaactgc, aggagattgcggtgacattg, attggatgaccagaaggctc, tgttggttacttggaatcgc, aggttttgcatttcaagcga, atcctgaatcggatcttgtg, aagccaaggatttggcattt, aattgcactcgaactgacct, gcttggtcaaattcttctct, ttgcatgtgaggcacttatg, tcatgtgcatcaggaggaag, aaagcaatcccgtgtttgac, tttggatattcgggatcagc, cggatacaggcgaatctaca, cgacattggggagacaggaa, agcgttgaatattcgtgtgc, cgtaaaacccgattccaact, aagtggatatgtccgtttgg, agatccggattgaagtaggg, gccaagttcaacctataagt, gataccccaagcttcttaaa, tggctatcttattagctcga, catagtcagaagctccatga, cttgcgagtgcgatatgtat, tcgagatccagatccaaagt, caagaagtttggggctttgg, atgctcagcgtttttagttt, agcgtccgagtgcttaaaat, gcgtttctttatttccattt	Stellaris smFISH probes	N/A
Shu gcgtttgagaaaagagcttt, agttggcgctattcttttgg, aaacgaattccggaatgcgc, gttttcttccattatcgcaa, ttgagcaactgtggggtgta, gatccgaataggacaaggga, aactcaacgccctttttcac, catggttctgctgcgaattg, tatgtccacggtgaacgtag, cgcacgcagttcgtcgaatg, tttcgtcgatctcgctcatc, cgagtgatccgcttgtaaat, cagtagccactgtaccgaac, cagggaggagtcgaagggag, tggccagtttcaaacacgaa, taaggacgcatgctgcggac, ggagattataaactcggcct, aaccgagttctccgaaaagc, cactttaaagagcgcatccg, cctatgagcgagtagtcaat, cttgggggatagcatcgatg, cacacaaaacttgtcgcggt, atgtaggtgcaagtcgacag, cagttgctgcactctgatag, cagtagttcagtgagctcac, agtaagcagttcagtttgct, gatcatcaggttttggttca, gcttgttcatcttgttgtaa, gtaaggccttcatcatgatg, gaagagcgccttgcaagacg, caagttgtactcgcccagag, ataatctcgtcgctgatctc, ctcatacttgctgattctct, tttaacgagaaggcacgtgc, tttgcggacgtctgactttg, ttgaaatcctgttcctttgc, gttcttaaaccgcctaatca, aagctcacttgttgatctga, gtcgaactgagcgttcgaat, ttccttggccaatttgcaag, gggagacaaagttagcttca, atgtcagaacatcctcttgg, aagcgaacttaacatccggc, tactggggcttcttagtaag, ctgctcttctagaacttgga, taacattcgtctgggcttac, acttaaggtctacgttgctc, gccgtgcattacttttattg	Stellaris smFISH probes	N/A
sra acgcattgttgttggacttg, gtcaggagtggtcggatcag, ttgtcggagtggctgcattg, cagtttgttcttgcttctgc, cactgctgttctgtgtcgac, cgacagcttgtctatgctgc, ggcggcgttgatgaaaatgt, ggagcgacggatgctgattg, tcgtcgaacgagtctgcatc, cacgatgattgaggtgggta, aaacacctcggagtggatgt, cgtgcttcagctctggattg, gaatgtgcggaataactcct, cactggaacgtagccgattc, aacacgcaggcgacggaagc, ccgcaatggcattgtcatag, cagcttgattcgcgcatttg, ccgtcttcttgttaaactcg, tgggcaaagtagcaggtgat, tgcaggttcttgttgctgac, ggggagatgaggaactgttt, caacgcggccaaaagatcat, atggccgtgtgcacaataat, cacttggtctggacaattgg, tcaccaccttatgattatgc, caggttttcagctttagttt, ccgatcgtttggttttacaa, aaatactcaatcgaagcccc, gttcgagtgcctattaatac, ggaaagtatgtcgggtatgg, atgctacgcttgaaggaagg, tgtatcagaagccaactggg, tgcggttgttagccaatgaa, atcgcgagataaacacatct, tcttacacatagacacactc, ttgcgatggcttaacatcaa, tgtagatattgttagcaggt, cgtcgaaggtacacagagat, atatctgggatttcggatcg, tcgctggcttagaattacat, gatgcgatatcgttacacga, gtatgtatgtgtatgccgat, actacacgtaggcatataga, ttcattttgttttgtttggt	Stellaris smFISH probes	N/A
Pi3K21B aagcccatctgcaatatcat,tcataaagttgctgccactt, tttagcaaccagagagcttc, ctctcgctcgaatcaggaag, atggacagagcatagtgacc, agtgctgcacgatgttcttg, ggtgctcgtctcgtaaataa, gtaaggggcagcaaatccaa, tcttcagcgtggcatatata, gaattgttggcatagtgctc, ttcttccagtacaagacagg, gatcatctgaacttgcagcg, tcgtactccagatccatttc, catctacgacgtcatgttca, agttggagggcgaaatggag, ttagcggcaatttgatgtgc, gagatagatagctagcctga, gagttagggctttagagcaa, cagccgtgtttatatctaga, cttttgacagagcctgcaaa, tactggcatatccagcttaa, gtctgactttcgagtcattc, cgctttactagcttgatgtt, atctgaatacatgttagggt, attggcagttcggacactaa, ttgtgacaaggagccttttt, ctttggctctcattactcaa, taacactaggtaacgcgtgt, ggccgaaataatagcattgc, tgctgatgtacacaatcggc, gccatccatcacacacaaaa, aattcttgaggggatagcgg, ttttcttttgtttctggagc, atcaccgaaaacgttttccc, agcttctagcaagctgttaa, atcacgcgaaatgcagtatt, acagatagctcgacttatca, atacgggatagggcgttgat, aatttcttcacgatcctacc, acacgacgaaatgtgctgaa, tgcttctttgtttcgttttt, tcgcaaacgttttgcaagcg, cggctaggttatcaaaacct, atattagagcaaggggtggg, acgtacgtagatttcactta, atcaactgatcacatcgagt, tttgttccgactgagtgtat, tctgcgtgtaaagttctctt	Stellaris smFISH probes	N/A
IRFP670-ORF cgatcacaggatgtgaggtc, tggaacctggtatgtggata, taatgcgtactgcctgtgca, cctgcattttctgtgatgcg, tgtctcccttccaaagaatg, cgagcaattcgccaacacgg, tccgtctcgccaaaataatc, agtgcgttacggagagcgtg, cagccaaatatgagggcagg, aaaagtcctgccagtcaatc, catggcggtgcaaactgatg, cggctcaaactctatgatgc, caatgatttgccgagtcagc, atctcctccaacgacttgag, catagcctggagatagcgtg, cgaagcgatagagcatcacg, cttttagcttcgccgattac, caggaacgattccaggtcac, ttcaaatacagcagacgggc, tgtcggaaaccacacggatc, acaatgcgcgaactgattcc, aactcaaatccagagctgcg, ccaaatgacacgggcttatc, tacgcccatatttcgcaaaa, tatggacagggacatgcttg, cccacaaggtaccatctata, gttcgtagtgatggcatatt, tacacgttgcgccattggaa, aaatccgcaaacatctctgc, ggccgtgaaatgcaaggaca	Stellaris smFISH probes	N/A
MS2 catgaggatcacccatgtct, agtattcccgggttcattag, cctaaggtacctaattgcct	Stellaris smFISH probes	N/A
EGFP tcctcgcccttgctcaccat, gggcaccaccccggtgaaca, cgccgtccagctcgaccagg, ctgaacttgtggccgtttac, gccctcgccctcgccggaca, tcagcttgccgtaggtggca, gtggtgcagatgaacttcag, ccagggcacgggcagcttgc, tcagggtggtcacgagggtg, ctgaagcactgcacgccgta, cttcatgtggtcggggtagc, cggacttgaagaagtcgtgc, tggacgtagccttcgggcat, cttgaagaagatggtgcgct, gggtcttgtagttgccgtcg, ccctcgaacttcacctcggc, gatgcggttcaccagggtgt, ttgaagtcgatgcccttcag, ccccaggatgttgccgtcct, tgtagttgtactccagcttg, catgatatagacgttgtggc, tgccgttcttctgcttgtcg, cggatcttgaagttcacctt, gctgccgtcctcgatgttgt, tggtagtggtcggcgagctg, gtcgccgatgggggtgttct, ttgtcgggcagcagcacggg, ggactgggtgctcaggtagt, cgttggggtctttgctcagg, aggaccatgtgatcgcgctt, ggcggcggtcacgaactcca, tcgtccatgccgagagtgat	Stellaris smFISH probes	N/A
osk, nos, CycB, gcl and GFP probes were described previously	(Trcek et al., 2015)
STORM smFISH Probes (5′ to 3′)
nos dSTORM3:ggtaaagctacgcgccaact (Alexa647; 5′UTR)	IDT Technologies	N/A
nos dSTORM4: aagcacagtttattcaactg (Alexa647; 5′UTR)	IDT Technologies	N/A
nos dSTORM5,7: ccaggcgctatttaaacgtt (Alexa 647; 3′UTR)	IDT Technologies	N/A
nos dSTORM6, 8: ttttcagaatatgtgtacac (Alexa647; 3′UTR)	IDT Technologies	N/A
nos dSTORM9, 10: cgagattggtggacacagtg (Alexa568; middle ORF)	IDT Technologies	N/A
nos dSTORM11:gtttccctttcacagaaaca (Alexa647; 3′UTR)	IDT Technologies	N/A
nos dSTORM12: tgatacgattgacagttcga(Alexa647; 3′UTR)	IDT Technologies	N/A
gcl dSTORM1: gggacagtaattacatgcgt (Alexa568; 5′UTR) (cross-correlation)	IDT Technologies	N/A
gcl dSTORM4: acttgtaaaactgcagttac (Alexa647; 5′UTR)	IDT Technologies	N/A
gcl dSTORM6: gcggatatgcttatactcga (Alexa647; 3′UTR)	IDT Technologies	N/A
gcl dSTORM7: cgaactgctgcgggtaaatg (Alexa647; 3′UTR)	IDT Technologies	N/A
gcl dSTORM8: ccgacgaatgttcagtctac (Alexa647; middle ORF)	IDT Technologies	N/A
gcl dSTORM9: tggaaccaaagacagcatcc (Alexa647; beginning ORF)	IDT Technologies	N/A
osk dSTORM1: aatcgcgcaaatgcttcact (Alexa647; 3′UTR, next to the stem loop)	IDT Technologies	N/A
osk dSTORM2: caataacttgcagtacgcgc (Alexa647; end of ORF)	IDT Technologies	N/A
osk dSTORM3: gatctgaaccaaaggcttgc (Alexa568; middle ORF)	IDT Technologies	N/A
osk dSTORM4: ggaattcacttgtgactgcg (Alexa568; 5′UTR)	IDT Technologies	N/A
osk dSTORM5: caggaaatccgtcacgttgt (Alexa647; beginning ORF)	IDT Technologies	N/A
osk dSTORM6: attacggccaaaatgcagca (Alexa647; 3′UTR next to the stem loop)	IDT Technologies	N/A
Osk dSTORM7: tacacagcttttgggatagc (Alexa647; 3′UTR)	IDT Technologies	N/A
CycB dSTORM3: gtttttgtatgaatgtgcga (Alexa647; 3′UTR) (cross-correlation)	IDT Technologies	N/A
qRT-PCR oligonucleotides (5′ to 3′)
nos Fw: acctacgtgtgccccatctg	IDT Technologies	N/A
nos Rv: ttccgccttgatcgcatcct	IDT Technologies	N/A
gcl Fw: tgactctgagccttcgacgc	IDT Technologies	N/A
gcl Rv: cgccagttgtccgcagattg	IDT Technologies	N/A
CycB Fw: cgcccactctgaccttctac	IDT Technologies	N/A
CycB Rv: ttggaccgcactatttcctc	IDT Technologies	N/A
DMN Fw: agacgcctggaagtaagcag	IDT Technologies	N/A
DMN Rv: gtaaggcggctcaacttgtc	IDT Technologies	N/A
Recombinant DNA
IRF670	Addgene	45457
Gal4-responsive UASp promoter plasmid	(Rorth, 1998)	N/A
Software and Algorithms
https://www.biosearchtech.com/stellarisdesigner	LGC Biosearch technologies	N/A
Airlocalize spot detection algorithm	(Trcek et al., 2017, Lionnet et al., 2011)
PCC(Costes) co-localization ImageJ plugin	(Bolte and Cordelieres, 2006)	JACoP ImageJ
Sigmaplot	www.systatsoftware.com	N/A
Script for pair-correlation analysis	(Veatch et al., 2012)	Matlab
Script for auto-correlation analysis	(Sengupta et al., 2011)	Matlab

Dataset has been submitted: Trcek, Tatjana (2020), “nanos mRNA and Vasa:GFP”, Mendeley Data, V1, http://dx.doi.org/10.17632/h4wfnfsgw8.1

https://data.mendeley.com/datasets/h4wfnfsgw8/draft?a=8f68add2-225c-4566-b69b-058167f2ce6d