Table 2.
Method validation criteria, sensitivity, accuracy, and precision (inter-day) at two quality control concentrations (5 and 50 ng/mL), calculated both without and with IS, for each compound added to water.
| Water | LoD* | LoQ* | Accuracy% | Precision% | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| QC1 | QC2 | QC1 | QC2 | |||||||
| Compound | ng/mL | ng/mL | No IS | With IS | No IS | With IS | No IS | With IS | No IS | With IS |
| MEPH | 25.4 | 84.5 | 90.9 | 101.0 | 102.0 | 106.0 | 4.4 | 5.6 | 4.2 | 4.3 |
| JWH200 | 55.7 | 185.0 | 91.6 | 102.0 | 103.0 | 106.0 | 4.0 | 5.3 | 2.8 | 3.0 |
| AM2201 | 1.8 | 6.0 | 85.0 | 94.5 | 97.9 | 101.3 | 3.1 | 4.4 | 3.0 | 2.5 |
| JWH250 | 378.0 | 1259.0 | 99.5 | 111.0 | 103.0 | 107.0 | 5.6 | 5.8 | 0.9 | 1.5 |
| CP47497-C7 | 831.0 | 2769.0 | 99.0 | 103.0 | 9.8 | 10.0 | ||||
| CP47497-C8 | 599.0 | 1965.0 | 146.0 | 162.0 | 19.0 | 19.0 | ||||
| CBD | 90.1 | 300.0 | 85.6 | 95.3 | 94.5 | 97.8 | 12.6 | 13.5 | 5.0 | 5.5 |
| THCCOOH | 190.0 | 635.0 | 85.7 | 96.0 | 100.0 | 105.0 | 2.5 | 2.8 | 3.9 | 4.6 |
| JWH081 | 0.9 | 2.9 | 87.8 | 97.6 | 107.0 | 111.0 | 13.1 | 13.4 | 12.3 | 12.3 |
| JWH122 | 1.1 | 3.6 | 88.9 | 98.9 | 106.0 | 110.0 | 4.7 | 5.4 | 2.0 | 2.6 |
| JWH019 | 1.5 | 5.0 | 92.0 | 102.0 | 106.0 | 110.0 | 4.2 | 5.7 | 3.4 | 3.3 |
| UR144 | 1.4 | 4.6 | 86.4 | 96.1 | 100.0 | 104.0 | 4.2 | 5.5 | 2.3 | 2.7 |
| CBN | 103.0 | 343.0 | 87.9 | 97.8 | 94.8 | 98.1 | 7.2 | 7.9 | 6.1 | 6.6 |
| HU221 | 76.6 | 255.0 | 94.0 | 105.0 | 92.9 | 96.3 | 2.9 | 1.3 | 2.3 | 2.5 |
| THC | 102.0 | 342.0 | 86.2 | 95.9 | 94.8 | 98.1 | 2.4 | 4.0 | 2.3 | 2.8 |
LOD*, Limit of detection x10−3, S/N = 3; LOQ*, Limit of quantification x10−3, S/N = 10; QC1, 5 ng/mL; QC2, 2, 50 ng/mL; No IS, results obtained with calibration curves constructed by linear regression analysis of the analyte area vs. the concentration of analytes injected; With IS, results obtained with calibration curves constructed by linear regression analysis of the ratio of the analyte area to the IS area vs. the concentration of analytes injected.