Figure 1.
Microenvironmental effect on cellular energy balance. (A) Experimental setup for differential oxygenation of primary rat hepatocytes. Gas-permeable culture plates are used for high [+] oxygen flux. Selective blocking with polyester seals [–] limits gas diffusion to the medium side only. Further variation of oxygen tension is achieved by multi-gas incubators with ambient pO2 set to 20, 10, 5, and 2.5%. (B) Cellular oxygen consumption rates (OCR) calculated from pericellular oxygen measurement. High flux conditions allow OCR in the range of previously reported in vivo values (Place et al., 2017) while blocked cultures are only viable in 20 and 10%. (C) Purine triphosphate balance and (D) pyrimidine triphosphate balance in conditions subjected to differential oxygenation. (E) Supernatant glucose and lactate concentration change per day on D5.