Figure 2.

Quantification of intracellular carbon energy metabolism. (A) Immunofluorescent staining of SLC2A1 expression in different oxygen conditions. Translocation to the plasma membrane indicates active glucose transporter function (scale bar = 10 μm). (B) Residual supernatant insulin concentration after 24 h of culture at day 5. (C) Simplified carbon energy metabolism showing phenotype-specific metabolic patterning. High flux periportal-like cultures exhibit increased gluconeogenetic capabilities, whereas pericentral and hypoxic cultures accumulate citric acid cycle intermediates. Low flux cultures ambiguously exhibit cytosolic metabolism with PKM2-dependent pentose phosphate pathway activation.