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Oxygen-dependent signaling induces zonation-like phenotypes. Immunofluorescent imaging of (A) CDH2, (B) active β-catenin (CTNNB), and (C) glutamine synthetase (GLUL) observed in different conditions (scale bar = 10 μm). (D) Intracellular glutamine levels and (E) secreted albumin levels after 5 days of differential oxygenation. (F) Comparative metabolomic overrepresentation analysis of untreated cultures after 5 days of culture.
