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. 2020 Jun 12;8:429. doi: 10.3389/fcell.2020.00429

FIGURE 3.

FIGURE 3

LINC01134 promotes HCC liver metastasis and lung metastasis in vivo. (A–C) LINC01134-stably-overexpressed and control HCCLM3 cells were intrasplenically injected into nude mice. At the 28th day after injection, the number (A) and size (B) of liver metastatic nodules were detected by HE staining (C). (D–F) LINC01134-stably-silenced and control HCCLM3 cells were intrasplenically injected into nude mice. At the 28th day after injection, the number (D) and size (E) of liver metastatic nodules were detected by HE staining (F). (G) Luciferase-labeled LINC01134-stably-overexpressed and control HCCLM3 cells were injected into the tail veins of nude mice. At the 28th day after injection, lung metastasis was assessed via detecting luciferase signal intensities. (H) Luciferase-labeled LINC01134-stably-silenced and control HCCLM3 cells were injected into the tail veins of nude mice. At the 28th day after injection, lung metastasis was assessed via detecting luciferase signal intensities. Results are shown as the mean ± SD of n = 6 mice in each group. **p < 0.01 by Mann–Whitney test (A–C,G) or Kruskal–Wallis test followed by Dunn’s multiple comparisons test (D–F,H).