FIGURE 5.

Profile of the galacto- and prenyl-lipids in Lepidium sativum grown at different temperatures. Total lipids were extracted from pooled leaf samples, separated by liquid chromatography and identified by mass spectrometry. Eleven leaf samples from three independent growth replicates were used. Relative concentration of the principal antioxidants of the thylakoid membrane (A) α-tocopherol, (B) γ-tocopherol, (C) plastochromanol, and its precursors (D) plastoquinone, the latter also serves as an electron carrier in the electron transport chain. In the box plots, the lower and upper hinges correspond to the first and third quartiles (the 25th and 75th percentiles). The whiskers extend from the hinge to the farthest value no further than 1.5 times the distance between the first and third quartiles from the hinge. Farther points are considered as “outliers” and plotted individually. The p-value derived from a post hoc test comparing the data with the value at 22°C is shown above each box. (E) Profile of the monogalactosyldiacylglycerols (MGDG) and digalactosyldiacylglycerols (DGDG) expressed as % molecules over the total of the measured galactolipids. The compounds are listed by the length and degree of saturation of the acyl chains, and significant differences from the control temperature 22°C (p < 0.05) are highlighted with an asterisk (n = 11).