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. 2020 Jun 4;11:1085. doi: 10.3389/fimmu.2020.01085

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Copyright © 2020 De Trez, Stijlemans, Bockstal, Cnops, Korf, Van Snick, Caljon, Muraille, Humphreys, Boon, Van Ginderachter and Magez.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cellular characterization of IL-10 producing cells in spleen and liver following T. brucei infection. (A) Percentages and (B) total cell number of IL-10 producing cells in infected liver (LI inf) and spleen (SP inf) at day 3, 6, and 8 p.i. (C) Relative contribution of the different cellular sources producing IL-10. The surface of the diagram is representative of the total number of IL-10⁺ cells present in liver and spleen at day 6 and day 8 p.i., which is described in (B). (D) Histogram showing the intensity of IL-10-eGFP signal as well as the percentage and MFI of IL-10⁺ cells in different cell subsets. The red dotted line is giving an indication of the relative IL-10-eGFP signal differences between the different cell subsets, whereas the gray histogram represent the relative IL-10-eGFP signal of these different cell subsets in infected non-reporter mice. Data are represented as mean of at least three mice per group ± SEM, where *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, and are representative of 3 independent experiments.