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. 2020 Jun 10;8:601. doi: 10.3389/fbioe.2020.00601

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Copyright © 2020 Naqvi, Panadero Pérez, Kumar, Verbruggen and McNamara.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Constructs under both continued estrogen treatment and estrogen withdrawal (EW) under static conditions and mechanical stimulation. Gene expression of BSP (A), OPN (B), RANKL (C), and OPG (D). Ratio of expression between RANKL and OPG (E). All constructs normalized to constructs of day 1 and E, ^#significance compared to static for the same experimental group and time point, ^&significance compared to earlier time point(s) for the same experimental group, p < 0.05 ***significance compared to E for the same experimental group and time point; p < 0.001, *significance compared to E for the same experimental group and time point; p < 0.05.