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. 2020 Jun 2;11:1077. doi: 10.3389/fimmu.2020.01077

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Copyright © 2020 Borsci, Barbieri, Guardamagna, Lonati, Ottolenghi, Ivaldi, Liotta, Tabarelli de Fatis, Baiocco and Savio.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Average relative changes in PBMC subset populations for all donors. (A) For PBMCs cultured alone, relative changes in CD4+, CD8+, CD19+, and CD56+ subsets as a function of time with respect to the 1 h time-point. For PBMCs co-cultured with both non-irradiated and 10 Gy-irradiated Caco-2 cells, relative changes with respect to PBMCs cultured alone at the same time-point for subsets: (B) CD4+; (C) CD8+; (D) CD19+; and (E) CD56+. Ratios are calculated for individual donors (excluding outliers identified in Figure 3) and then averaged. Errors are obtained as standard deviations. Statistical significance (p < 0.05) is calculated by two-tailed Student's t-test.