Figure 5.

Principal Components Analysis for discrimination of donors for different experimental conditions. (A) PCA applied to lymphocytic subsets for each individual donor for: PBMCs cultured alone (squares); PBMCs in co-culture with sham-irradiated Caco-2 (stars); and PBMCs in co-culture with 10 Gy-irradiated Caco-2 cells (filled dots). (B) PCA applied to data at a fixed 72 h time point expressed as ratios between subset percentages of PBMCs when in co-culture with non-irradiated (star) or 10 Gy-irradiated Caco-2 cells (filled dots) and the same subset percentages when PBMCs are cultured alone. Dashed lines are drawn to guide the eye and connect points representing the same donors in different experimental conditions. Donors are identified by their numbers, the label is positioned close to the point representing PBMCs alone in (A) and the co-culture with non-irradiated Caco-2 cells in (B).