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. 2020 May 21;11:718. doi: 10.3389/fphar.2020.00718

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Copyright © 2020 Zhang, Cao, Yang, Fang, Yang, Li, Hong and Ji

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Bilobalide inhibited differentiation of M1 macrophage. (A, B) Bone marrow-derived macrophages (BMDMs) were treated with 1, 3, and 10 μM bilobalide in the presence of 10 ng/ml lipopolysaccharide (LPS) and 10 ng/ml interferon-gamma (IFN-γ) (M1) for 12 h. The expressions of major histocompatibility complex II (MHC-II) and CD11c were analyzed through flow cytometry. Data was summarized as a histogram of mean ± SEM of three independent experiments. *P < 0.05 and **P < 0.01 compared with LPS and IFN-γ group.