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. 2020 May 27;11:980. doi: 10.3389/fimmu.2020.00980

Figure 4.

Figure 4

TNF-α increases c-Jun mRNA and protein expression. HCT116 CRC cells were treated with increasing concentrations of TNF-α (A,B) or IFN-γ (C,D) for 24 (left panels) and 48 h (right panels). Cells without TNF-α treatment served as controls. The mRNA and protein expression levels of c-Jun and Sp1 were measured by qRT-PCR. Western blot was used to confirm the upregulation at the mRNA level. TNF-α treatment induces c-Jun expression at the mRNA and protein level. Results are representative of at least three independent experiments. The data are presented as mean ± SEM with the statistical significance levels: ***p ≤ 0.001 and ****p ≤ 0.0001.