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. 2020 May 27;11:645. doi: 10.3389/fpls.2020.00645

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Copyright © 2020 Galán-Ávila, García-Fortea, Prohens and Herraiz.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Direct in vitro shoot organogenesis from true leaves of C. sativa. The different developmental stages are described as follows: (A) Newly dissected leaf from a 7-days-old hemp seedling. (B) Formation of one primordium from leaf-petiole transition zone 1 week after culture initiation. (C) Two-week-old plantlet of approximately one centimeter in height ready for subculture. (D) Leaf derived plant individually grown in a glass-tube 21 days after culture initiation. Scale bars (A–C): 1 mm. Scale bar (D): 6 mm.