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. 2020 May 27;11:645. doi: 10.3389/fpls.2020.00645

TABLE 2.

Effect of genotype, explant and medium on direct in vitro shoot organogenesis rate of different explants from C. sativa.

Responding
Factor explants (%) Significancea n
Variety
Ferimon 6.23 ± 1.06 bc 514
Felina32 7.37 ± 1.03 bc 638
Fedora17 8.55 ± 1.36 ab 421
USO31 12.32 ± 1.61 a 414
Finola 4.62 ± 0.96 c 476
Explant
Cotyledon 4.70 ± 0.66 b 1000
Hypocotyl 49.45 ± 3.02 a 275
Leaf 0.42 ± 0.18 c 1188
Medium (mg/L)
0 → Without plant growth regulators 6.81 ± 1.24 b 411
1 → TDZ 0.4 + NAA 0.2 15.78 ± 1.79 a 412
2 → BAP 2+ IBA 0.5 4.61 ± 1.25 b 282
3 → BAP 0.5 + 2,4-D 0.1 6.42 ± 1.50 b 265
4 → ZEARIB 2 6.01 ± 1.76 b 183
5 → BAP 1 + NAA 0.02 5.50 ± 1.54 b 218
6 → BAPRIB 1 + NAA 0.02 6.71 ± 1.95 b 164
7 → TDZ 1 + NAA 0.02 4.37 ± 1.51 b 183
8 → 4-CPPU 1 + NAA 0.02 8.67 ± 2.30 b 150
9 → ZEARIB 1 + NAA 0.02 5.13 ± 1.58 b 195

Mean of responding explants (%), significance and sample size (n) are presented in different columns. For each factor, mean of responding explants is expressed as a percentage (±SE) relative to the total amount of cultured explants. aDifferent letters among the levels of each of the three factors indicate significant differences between them (p < 0.05) according to non-parametric Kruskal-Wallis and pairwise Wilcoxon tests.