TABLE 2.
Effect of genotype, explant and medium on direct in vitro shoot organogenesis rate of different explants from C. sativa.
Responding | |||
Factor | explants (%) | Significancea | n |
Variety | |||
Ferimon | 6.23 ± 1.06 | bc | 514 |
Felina32 | 7.37 ± 1.03 | bc | 638 |
Fedora17 | 8.55 ± 1.36 | ab | 421 |
USO31 | 12.32 ± 1.61 | a | 414 |
Finola | 4.62 ± 0.96 | c | 476 |
Explant | |||
Cotyledon | 4.70 ± 0.66 | b | 1000 |
Hypocotyl | 49.45 ± 3.02 | a | 275 |
Leaf | 0.42 ± 0.18 | c | 1188 |
Medium (mg/L) | |||
0 → Without plant growth regulators | 6.81 ± 1.24 | b | 411 |
1 → TDZ 0.4 + NAA 0.2 | 15.78 ± 1.79 | a | 412 |
2 → BAP 2+ IBA 0.5 | 4.61 ± 1.25 | b | 282 |
3 → BAP 0.5 + 2,4-D 0.1 | 6.42 ± 1.50 | b | 265 |
4 → ZEARIB 2 | 6.01 ± 1.76 | b | 183 |
5 → BAP 1 + NAA 0.02 | 5.50 ± 1.54 | b | 218 |
6 → BAPRIB 1 + NAA 0.02 | 6.71 ± 1.95 | b | 164 |
7 → TDZ 1 + NAA 0.02 | 4.37 ± 1.51 | b | 183 |
8 → 4-CPPU 1 + NAA 0.02 | 8.67 ± 2.30 | b | 150 |
9 → ZEARIB 1 + NAA 0.02 | 5.13 ± 1.58 | b | 195 |
Mean of responding explants (%), significance and sample size (n) are presented in different columns. For each factor, mean of responding explants is expressed as a percentage (±SE) relative to the total amount of cultured explants. aDifferent letters among the levels of each of the three factors indicate significant differences between them (p < 0.05) according to non-parametric Kruskal-Wallis and pairwise Wilcoxon tests.