TABLE 3.

Effect of genotype and medium on direct in vitro shoot organogenesis rate of cotyledons from C. sativa.

Factor	Responding explants (%)	Significancea	n	Shoots per responding explant	Significancea	n
Variety
Ferimon	5.50 ± 1.61	ab	200	1.09 ± 0.09	a	11
Felina32	2.10 ± 0.84	c	286	1.17 ± 0.17	*	6
Fedora17	6.67 ± 1.86	ab	180	1.42 ± 0.15	a	12
USO31	9.29 ± 2.46	a	140	1.00 ± 0.00	a	13
Finola	2.58 ± 1.14	bc	194	1.00 ± 0.00	*	5
Medium (mg/L)
0 → Without plant growth regulators	0.00 ± 0.00	d	234	–	*	0
1 → TDZ 0.4 + NAA 0.2	22.32 ± 3.95	a	112	1.28 ± 0.09	a	25
2 → BAP 2 + IBA 0.5	1.85 ± 1.30	c	108	1.00 ± 0.00	*	2
3 → BAP 0.5 + 2,4-D 0.1	5.56 ± 2.42	bc	90	1.00 ± 0.00	*	5
4 → ZEARIB 2	1.28 ± 1.28	cd	78	1.00	*	1
5 → BAP 1 + NAA 0.02	1.92 ± 1.35	c	104	1.00 ± 0.00	*	2
6 → BAPRIB 1 + NAA 0.02	6.25 ± 3.04	bc	64	1.00 ± 0.00	*	4
7 → TDZ 1 + NAA 0.02	2.56 ± 1.80	c	78	1.00 ± 0.00	*	2
8 → 4-CPPU 1 +NAA 0.02	14.29 ± 5.46	ab	42	1.00 ± 0.00	a	6
9 → ZEARIB 1 + NAA 0.02	0.00 ± 0.00	d	90	–	*	0

Mean of responding explants (%), significance and sample size (n) are presented in different columns. For each factor, mean of responding explants is expressed as a percentage (±SE) relative to the total amount of cultured explants. This table also includes the effect of genotype and medium on the number of shoots per responding cotyledon of C. sativa. Mean number of shoots per responding explant (±SE), significance and sample size (n) are presented in different columns. Shoots per responding explant from varieties and media with the best shoot induction rates are statistically compared. ^aDifferent letters among the levels of each of the two factors indicate significant differences between them (p < 0.05) according to non-parametric Kruskal-Wallis and pairwise Wilcoxon tests. * Not analyzed statistically.